Binding of CDK9 to TRAF2 |
| |
Authors: | Timothy K. MacLachlan Nianli Sang Antonio De Luca Pier Lorenzo Puri Massimo Levrero Antonio Giordano |
| |
Affiliation: | 1. Sbarro Institute for Cancer Research and Molecular Medicine, Department of Pathology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107;2. Department of Biology, University of California-San Diego, La Jolla, California 92093;3. Laboratory of Gene Expression, Fondazione Andrea Cesalpino, University of Rome, La Sapienza, 00161 Rome, Italy |
| |
Abstract: | CDK9 has been recently shown to have increased kinase activity in differentiated cells in culture and a differentiated tissue-specific expression in the developing mouse. In order to identify factors that contribute to CDK9's differentiation-specific function, we screened a mouse embryonic library in the yeast two-hybrid system and found a tumor necrosis factor signal transducer, TRAF2, to be an interacting protein. CDK9 interacts with a conserved domain in the TRAF-C region of TRAF2, a motif that is known to bind other kinases involved in TRAF-mediated signaling. Endogenous interaction between the two proteins appears to be specific to differentiated tissue. TRAF2-mediated signaling may incorporate additional kinases to signal cell survival in myotubes, a cell type that is severely affected in TRAF2 knockout mice. J. Cell. Biochem. 71:467–478, 1998. © 1998 Wiley-Liss, Inc. |
| |
Keywords: | cell cycle kinase signal transduction differentiation |
|
|