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Mutants of the cyanobacterium Synechocystis PCC6803 impaired in respiration and unable to tolerate high salt concentrations
Authors:Robert Jeanjean  B. Onana  G.A. Peschek  F. Joset
Affiliation:Unitéde Métabolisme Energétique, LCB, BP3, 13275 Marseille cedex, France;Biophysical Chemistry Group, Institute of Physical Chemistry, University of Vienna, 1090 Vienna, Austria
Abstract:Abstract The cyanobacterium Synechocystis PCC6803, when challenged with a salt stress (0.5 M), increased its respiratory and cytochrome c oxidase activities. Spontaneous mutants impaired in respiration could be isolated through their incapacity to tolerate salt stress. Mutants from one class, among two classes obtained, were deficient for respiratory capacity and cytochrome c oxidase activity. The latter enzyme exhibited similar kinetic modifications, but no change in the M r of subunits I and II, in the complexes present in the cytoplasmic and thylakoid membranes.
Keywords:Cyanobacteria    Synechocystis    Respiration    Salt stress    Mutant    Cytochrome c oxidase
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