Stable isotope probing identifies anthracene degraders under methanogenic conditions |
| |
Authors: | Shuying Zhang Qingfeng Wang Shuguang Xie |
| |
Institution: | (1) College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China; |
| |
Abstract: | Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in groundwater. The remediation of PAH-contaminated groundwater
often involves anaerobic biodegradation. The knowledge about the microorganisms responsible for PAH degradation in anaerobic
subsurface environment is still lacking. DNA-based stable isotope probing (SIP) was applied to discover the microorganisms
responsible for anaerobic anthracene degradation within microcosms inoculated with aquifer sediment from landfill leachate-contaminated
site. Three phylotypes were identified as the degraders, all falling within the phylum Proteobacteria. Two anthracene degraders were classified within the genera Methylibium and Legionella, while another one was an unclassified Rhizobiales species. They all were first linked to PAH degradation. These findings also provide an illustration of the utility of SIP
to discover the roles of uncultured microorganisms in PAH-degrading processes. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|