妊娠妇女与无性生活女性阴道分泌物 微生物组学分析

目的探讨妊娠妇女与无性生活女性阴道分泌物中菌群结构差异,为临床女性泌尿生殖系统疾病研究提供可靠的依据。方法运用高通量二代测序技术检测20例妊娠妇女和29例无性生活女性阴道分泌物中微生物;采用秩和检验进行组间显著性差异分析,使用生物信息学软件进行数据处理。结果 49份样本共注释出14个门、23个纲、33个目、28个科、38个属和23个种水平物种。妊娠妇女与无性生活女性阴道分泌物中菌群结构差异显著。妊娠妇女在种水平仅有Atopobium vaginae、Campylobacter ureolyticus、Lactobacillus coleohominis、德氏乳杆菌、瑞氏乳杆菌、惰性乳杆菌、罗伊乳杆菌、白假丝酵母菌和近平滑假丝酵母菌共9个物种。结论妊娠能引起女性阴道分泌物菌群结构发生改变,且菌种数量明显减少。Lactobacillus coleohominis和德氏乳杆菌可能在维持妊娠妇女阴道菌群平衡和自净作用中起到关键性作用。

Microbiological analysis of vaginal secretions in pregnant women and virgins

Abstract: Objective To investigate the differences of bacterial flora in vaginal secretions between pregnant women and virgins without sex, and provide a reliable basis for clinical research on female genitourinary diseases. Methods Twenty cases pregnant women and twenty-nine cases virgins without sex were detected by high throughput second-generation sequencing. Rank-sum test was used to analyze the significant difference between groups, and bioinformatics software was used for data processing. Results A total of 14 phylum, 23 classes, 33 orders, 28 families, 38 genera and 23 species were identified in 49 samples.There were significant differences in the microflora structure between the vaginal secretions of pregnant women and virgins without sex .There were only 9 species of Atopobium vaginae, Campylobacter ureolyticus, Lactobacillus coleohominis, lactobacillus delbrueckii, Lactobacillus helveticus, Lactobacillus inert, Lactobacillus reuteri, Candida albicans and Candida parapsilosis at the level of pregnant women. Conclusion Pregnancy can change the microflora of female vaginal secretion and reduce the number of bacteria. Lactobacillus coleohominis and Lactobacillus desilis may play a key role in maintaining the balance and self-purification of vaginal flora in pregnant women.