基于16S rRNA基因高通量测序的太原地区健康成年人肠道菌群结构分析

目的 探讨太原地区健康成年人的肠道菌群结构及多样性。方法 采集太原地区20份健康成年人的粪便样本，提取DNA、构建菌群16S rRNA基因克隆文库、高通量测序并进行生物信息学分析。结果 测序获得优质序列1 383 680条，平均序列为69 184条±551条，归类于455个OTUs；所有样本共含有10个菌门，101个菌属，141种菌；其中核心菌门3个，依次为拟杆菌门（63.13%）、厚壁菌门（31.14%）和变形菌门（5.10%），占所有样本微生物总量的99.37%；丰度最高的前10个菌属依次为拟杆菌属（43.34%）、普氏菌属（15.51%）、栖粪杆菌属（4.92%）、罗氏菌属（4.73%）、毛螺菌属（3.27%）、萨特菌属（2.50%）、粪球菌属（2.38%）、布劳特菌属（2.31%）、瘤胃球菌属（2.18%）和副杆状菌属（1.61%），占样本微生物总量的82.75%，未分类的菌属占6.84%。结论 健康成年人肠道微生物群落复杂，但主要菌群相对稳定，为进一步研究人体肠道菌群结构与功能提供了参考依据。

The gut microflora structure of healthy adults in Taiyuan area by 16S rRNA sequencing

Abstract: Objective To explore the structure and diversity of gut microflora of healthy adults in Taiyuan area. Methods Twenty cases fecal samples of healthy adults in Taiyuan area were collected, DNA of these fecal samples was extracted, the 16S rRNA gene clone library of bacteria was constructed, high-throughput sequencing was conducted, and bioinformatics analysis was conducted. Results High quality sequences were obtained(1,383,680), with an average of 69 184±551 sequences, which were classified into 455 OTUs. All the samples involved 10 phyla, 101 genera and 141 species of microorganisms. Bacteroidetes, Firmicutes and Proteobacteria were the core bacteria, accounting for 63.13%, 31.14% and 5.10% respectively. The top 10 genera were Bacteroides (43.34%), Prevotella (15.51%), Faecalibacterium (4.92%), Roseburia (4.73%), Lachnospira (3.27%), Sutterella (2.50%), Coprococcus (2.38%), Blautia (2.31% ), Ruminococcus (2.18%) and Parabacteroides (1.61%), accounting for 82.75% of the total microbial species, and 6.84% of unclassified species. Conclusion The gut microflora of healthy adults are complex, but the main microflora are relatively stable. This result provides a reference for further study on the structure and function of human gut microflora.