保和丸对食积小鼠的肠道微生物及酶活性的影响

目的 探究保和丸对食积小鼠肠道微生物及酶活性的影响。方法 将32只实验小鼠（雌雄各半）随机分为正常组（n=12）、食积组（n=10）和治疗组（n=10）。其中正常组喂食正常饲料，其余组喂食特制饲料。待食积组和治疗组中小鼠出现粪便干结，腹部膨隆胀满后，立即对治疗组按6.67 g/(kg·d)剂量灌胃0.28 g/mL的保和丸汤剂，正常组与食积组灌胃等量的蒸馏水。每天2次，每次0.35 mL，持续4 d。比较分析小鼠肠道微生物及肠道酶活性。结果 肠道微生物方面，食积组能显著增加细菌、乳酸菌、双歧杆菌和大肠埃希菌的数目，与正常组相比差异有统计学意义（均P0.05）。结论 保和丸能降低食积小鼠的细菌、乳酸菌以及大肠埃希菌的数目，同时能降低木聚糖酶、淀粉酶、蛋白酶和蔗糖酶等的活性。

Effect of Bao-he Pills on intestinal microorganisms and enzyme activity in mice with dyspepsia

Objective To explore the effect of Bao-he Pills on intestinal microorganisms and enzyme activity in mice with dyspepsia. Methods The experimental animals were randomly divided into control group(n=12), dyspepsia group(n=10) or treatment group(n=10). The control group was fed normal diet, and the other two groups were fed special diet. After dry stool and abdominal distension of mice in the dyspepsia group and treatment group， 0.28 g/mL of of Bao-he Pills decoction was given immediately through gavage at a dose of 6.67 g/(kg·d) to the treatment group, while the control group and dyspepsia group were given the same amount of distilled water by gavage, 0.35 mL bid, for 4 days. The intestinal microorganisms and intestinal enzyme activity were compared and analyzed. Results The amounts of bacteria, Lactic acid bacteria, Bifidobacteria and Escherichia coli in the dyspepsia group were significantly higher than those in the control group respectively (all P0.05). Conclusion Bao-he Pills can reduce the amounts of bacteria, Lactobacilli and Escherichia coli, and the activities of xylanase, amylase, protease and sucrase.