妊娠期糖尿病母亲哺乳期母乳N-聚糖的岩藻糖基化水平差异及其对新生儿肠道菌群的影响

目的 比较中国妊娠期糖尿病母亲与正常母亲母乳中N-聚糖的岩藻糖基化水平差异，分析这些差异对其子代肠道微生态的影响。方法 收集妊娠期糖尿病母亲和健康母亲在哺乳期第6天和第42天母乳样本各15例，及其纯母乳喂养的新生儿在同一天的粪便样本；以凝集素AAL分析母乳中N-聚糖的岩藻糖基化水平；以变性梯度凝胶电泳（DGGE）检测两组新生儿肠道菌群差异。结果 妊娠期糖尿病母亲母乳N-聚糖岩藻糖基化水平显著高于健康母亲(t=4.438，P<0.01；t=3.238，P<0.001)；两组新生儿肠道菌群存在明显差异，其中主要的微生物群如长双歧杆菌属、肠球菌属、泛菌属等的相对丰度有所不同。结论 母乳中N-聚糖岩藻糖基化水平在妊娠期糖尿病母亲母乳中显著升高，这一变化可能影响其子代的肠道菌群结构。

The difference in levels of fucosylated N-glycans in women withgestational diabetes mellitus and its effect on intestinal microbiota of breastfed infants

Objective To observe the difference in levels of fucosylated N-glycans between Chinese mothers with gestational diabetes mellitus (GDM) and healthy controls, and analyze the effects of these differences on the gut microbiota of their breastfed infants. Methods Milk samples from 15 cases mothers with gestational diabetes and healthy controls and stool samples of breast-fed newborns were collected on the 6th and 42nd day of lactation. The differences in the levels of fucosylated N-glycans in breast milk were analyzed by using lectin blot. The differences in neonatal gut microbiota between the two groups were detected using denatured gradient gel electrophoresis (DGGE). Results There was a significant difference in the levels of fucosylated N-glycans between the mothers with gestational diabetes mellitus and healthy mothers(t=4.438，P<0.01；t=3.238，P<0.001), and in the structure of intestinal microbiota of their newborns. Conclusion Compared with healthy mothers, the levels of fucosylated N-glycans in mothers with gestational diabetes mellitus is significantly higher, which may significantly affects the structures of the gut microbiota of their offsprings.