益生菌干预对肥胖小鼠肝脏miR-33和miR-122表达的影响

目的 探讨益生菌干预对高脂饮食诱导肥胖小鼠肝脏miR-33和miR-122表达的影响。方法 18只雌性C57BL/6J小鼠随机分为对照组、肥胖组和益生菌干预组，每组6只，分别给予标准饲料、高脂饲料以及高脂饲料＋益生菌合剂灌胃，自由采食及饮水，连续喂养6周。每周测量3组小鼠的体质量，6周后，留取小鼠血液样本采用全自动生化仪检测小鼠血脂，安乐法处死小鼠，留取小鼠肝脏样本Hair-pin RT-PCR法检测miR-33和miR-122的含量。结果 与对照组小鼠相比，肥胖组小鼠体质量明显增加（t2周=3.985，t3周=4.751，t4周=4.380，t5周=4.728，t6周=4.112，均P<0.01）；益生菌干预组小鼠体质量较肥胖组明显降低（t3周=3.694，t4周=4.415，t5周=3.752，t6周=3.392，均P<0.01）；肥胖组小鼠血清总胆固醇、低密度脂蛋白含量较对照组明显升高（t=10.850，t=7.024，均P<0.01），益生菌干预组小鼠较肥胖组小鼠血清总胆固醇、低密度脂蛋白含量降低（t=3.034，t=2.881，均P<0.05），但与对照组仍有差异。与对照组小鼠相比，肥胖组小鼠肝脏miR-122的表达升高（t=9.170，P<0.01），miR-33的表达降低（t=3.420，P<0.05），益生菌干预组小鼠较肥胖组小鼠miR-122的表达降低（t=3.204，P<0.05），miR-33的表达升高（t=2.070，P<0.05）。结论 益生菌干预能够影响高脂饮食小鼠肝脏miR-33和miR-122的表达，这可能是益生菌干预改善高脂饮食小鼠肝脏脂代谢的机制之一。

Effect of probiotic intervention on the expression of miR-33 and miR-122 inthe liver of high fat diet induced obese mice

Objective To explore the influence of probiotic intervention on the expression of miR-33 and miR-122 in the liver of high fat diet induced obese mice. Methods Eighteen female C57BL/6J mice were randomly divided into 3 groups: control group (lab chow), obesity group (high fat diet) or probiotic intervention group (high fat diet and probiotics), 6 mice in each group. During the experiment, all the mice were weighted weekly. After 6 weeks, the levels of serum lipids were detected by using automatic biochemical analyzer; The levels of miR-33 and miR-122 in mouse livers were detected with Hair-pin RT-PCR method. Results Compared with the control group, the weights in the obese group increased significantly （t2 week=3.985，t3 week=4.751，t4 week=4.380，t5 week=4.728，t6 week=4.112，all P<0.01）. The weights in the probiotics intervention group were significantly lower than in the obese group （t3 week=3.694，t4 week=4.415，t5 week=3.752，t6 week=3.392，all P<0.01）. The serum levels of total cholesterol and low density lipoprotein in the obese group were significantly higher than those in the control group (t=10.85，t=7.024，all P<0.01), while those in the probiotics intervention group were lower than those in the obese group (t=3.034，t=2.881，all P<0.05), but there were still differences between the two groups. Compared with the control group, the miR-122 level in the obesity group increased (t=9.170，P<0.01), while the miR-33 level decreased (t=3.420，P<0.05). Compared with the obesity group, the expression of miR-122 decreased （t=3.204，P<0.05）and that of miR-33 increased in the probiotics intervention group (t=2.070，P<0.05). Conclusion Probiotics intervention can affect the expression of miR-33 and miR-122 in the liver of high-fat diet mice, which may be one of the mechanisms of probiotics intervention in improving lipid metabolism in the liver of high-fat diet mice.