新疆博州地区三民族儿童口腔优势菌群结构

目的研究新疆博州地区汉族、维吾尔族和蒙古族3～5岁儿童的口腔唾液细菌优势菌群结构多样性的差异,并探讨与低龄儿童龋发生的相关性。方法从本课题组在新疆博州地区3～5岁儿童口腔流行病学调查时建立的资料样本库中选取唾液样本120例(汉族40例,维吾尔族40例,蒙古族40例)作为研究对象,其中每个民族中高龋组(ECC)各20例,无龋组(CF)各20例,取非刺激性唾液,提取细菌DNA后进行聚合酶链反应(PCR)扩增和变性梯度凝胶电泳(DGGE)分析,采用Quantity One软件对PCR-DGGE指纹图谱进行数据分析。结果 DGGE总体图谱表明:口腔微生物菌群结构具有民族差异性和个体差异性;维吾尔族总的条带数和多样性指数(条带数:87.00±10.40,多样性指数:5.99±1.12)大于汉族(条带数:63.00±8.07,多样性指数:3.29±0.74)和蒙古族(条带数:71.60±5.79,多样性指数:4.98±0.50)(P0.05),高龋儿童组的细菌菌群结构多样性小于无龋儿童组(P0.05),无龋儿童组的细菌菌群结构多样性存在民族差异(P0.05);聚类分析发现:每个民族组内大部分样本均表现出明显的聚类群。结论博州地区儿童口腔唾液细优势菌群结构多样性在民族之间差异明显,在低龄儿童龋病进展中,细菌多样性逐渐减少。

The dominant bacterial communities in the oral saliva ofchildren among three nationalities in Bozhou, Xinjiang

Abstract: Objective To explore the differences in the structural diversity of dominant oral salivary bacteria among 3 - 5 years old Mongolian, Uygur and Han children in Bortala (Bozhou), Xinjiang. Methods 120 saliva specimens from children aged 3 to 5 years (40 Hans, 40 Uighurs, 40 Mongolians) were randomly selected from the sample database established through the oral epidemiological survey in Bozhou, Xinjiang. In each ethnic groups, there were 20 severe early childhood caries (ECC) samples and 20 caries-free (CF) samples. The specimens of non-irritating saliva were collected, and bacterial DNAs were extracted for polymerase chain reaction (PCR) amplification and denaturing gradient gel electrophoresis (DGGE) analysis. Quantity One software was used to analyze the PCR-DGGE fingerprint. Results The DGGE profile showed that salivary bacteria had ethnic differences and intra-individual differences; the total band number and diversity index of Uygur population (band number: 87.00±10.40; diversity index: 5.99±1.12) were higher than those of Hans (band number: 63.00±8.07; diversity index: 3.29±0.74) and Mongolians (band number: 71.60±5.79; diversity index: 4.98±0.50) respectively (Ps<0.05). The structural diversity of bacterial community in ECC groups was less than in the CF groups (P<0.05). There were ethnic differences in the bacterial structures in the CF groups (P<0.05). The cluster analysis found most of the samples in each ethnic group showed obvious clusters. Conclusion The structural diversities of dominant oral bacterial community in the saliva of children in Bozhou area are significantly different among ethnic groups. During early stage of caries development, bacterial diversity in the saliva tends to decrease.