单核细胞趋化蛋白-1、高迁移率族蛋白B1与溃疡性结肠炎患者肠道菌群变化的相关性

目的 探讨单核细胞趋化蛋白-1（MCP-1）、高迁移率族蛋白B1（HMGB1）与溃疡性结肠炎（UC）患者肠道菌群变化的相关性。方法 选取2016年9月‒2018年1月遂宁市中心医院收治的98例UC患者资料，根据Mayo评分系统将UC患者分为活动期组（n=50）和缓解期组（n=48）。选取同期进行体检的健康人50例作为对照组。比较各组间肠道菌群，血清MCP-1、HMGB1水平，并进行Pearson相关分析。结果 活动期组患者肠道乳杆菌、双歧杆菌含量[（5.34±0.87）、（5.81±0.83）CFU/g]显著低于缓解期组和对照组[（8.07±0.86）、（8.35±0.88）CFU/g；（8.13±0.91）、（8.46±0.95）CFU/g]（F=12.035，P0.05）。活动期组和缓解期组患者血清MCP-1、HMGB1水平[（267.42±23.51）、（21.35±2.26）ng/mL；（188.15±20.73）、（6.28±1.38）ng/mL]显著高于对照组[（106.38±15.92）、（2.13±0.41）ng/mL]（F=84.163，P<0.001；F=25.386，P<0.001）；活动期组患者血清MCP-1、HMGB1水平[（267.42±23.51）、（21.35±2.26）ng/mL]显著高于缓解期组[（188.15±20.73）、（6.28±1.38）ng/mL]（t=17.676、39.641，均P<0.05）。经过Pearson相关性分析，MCP-1、HMGB1与UC患者乳杆菌、双歧杆菌含量呈负相关（r=‒0.715、‒0.659，r=‒0.703、‒0.614，均P<0.001），与大肠埃希菌、肠球菌、拟杆菌含量呈正相关（r=0.783、0.702，r=0.762、0.735，r=0.653、0.612，均P<0.001）。结论 MCP-1、HMGB1作为促炎因子可介导肠黏膜炎性反应，引起UC患者肠道菌群的紊乱。

The relationship between monocyte chemoattractant protein-1, high mobility group protein B1 and intestinal flora in patients with ulcerative colitis

Objective To investigate the correlation between monocyte chemoattractant protein-1 (MCP-1), high mobility group protein B1 (HMGB1) and intestinal flora in patients with ulcerative colitis (UC). Methods Ninety-eight UC patients admitted in our hospital from September 2016 to January 2018 were enrolled. According to Mayo scoring system, the UC patients were divided into active stage group (n=50) or remission group (n=48). 50 healthy physical examinees were selected as the control group. The intestinal flora and levels of serum MCP-1 and HMGB1 were compared between groups and Pearson correlation analysis was performed. Results The contents of Lactobacilli and Bifidobacteria in active stage group [(5.34±0.87)CFU/g, (5.81±0.83)CFU/g] were significantly lower[(8.07±0.86)CFU/g, (8.35±0.88)CFU/g; (8.13±0.91)CFU/g, (8.46±0.95)CFU/g](F=12.035, P0.05). The levels of serum MCP-1 and HMGB1 in active stage group and remission group[(267.42±23.51)pg/mL, (21.35±2.26)ng/mL; (188.15±20.73)pg/mL, (6.28±1.38)ng/mL] were significantly higher than those in control group respectively [(106.38±15.92)pg/mL, (2.13±0.41)ng/mL](F=84.163，P<0.001; F=25.386, P<0.001), and those in active group [(267.42±23.51)pg/mL, (21.35±2.26)ng/mL]were significantly higher than in remission group [(188.15±20.73)pg/mL, (6.28±1.38)ng/mL](t=17.676, 39.641，all P<0.05). By Pearson correlation analysis, MCP-1 and HMGB1 had a negative correlation with the contents of Lactobacilli and Bifidobacteria (r=-0.715, -0.659; r=-0.703, -0.614, all P<0.001), and a positive correlation with those of Escherichia coli, Enterococci and Bacteroides (r=0.783, 0.702; r=0.762, 0.735; r=0.653, 0.612, all P<0.001). Conclusion As proinflammatory factors, MCP-1 and HMGB1 mediate intestinal mucosal inflammatory response, and may lead to the disturbance of intestinal flora in patients with UC.