Identification of a surface epitope of human erythropoietin with anti-peptide antibodies |
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| Authors: | A J Sytkowski D M Wojchowski |
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| Institution: | 1. Duke Clinical Research Institute and Division of Cardiology, Durham, NC;2. Division of Cardiology, Duke University Medical Center, Durham, NC;3. Heart and Vascular Institute, Anchorage, AL;4. Department of Cardiovascular Diseases, Mayo Clinic, Rochester, MN;5. Division of Research, Kaiser Permanente Northern California, Oakland, CA;6. Division of Cardiology, Massachusetts General Hospital, Boston, MA |
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| Abstract: | Antibodies reactive with human erythropoietin were isolated from the serum of rabbits immunized with a twenty-six amino acid synthetic polypeptide corresponding to a proposed NH2-terminal sequence of the hormone. As shown by inhibition with peptide fragments, those antibodies that bound to erythropoietin recognized the (8-15) domain, strongly suggesting tht this region is exposed on the hormone's surface. This was confirmed by affinity purification of these antibodies on immobilized fragment (8-15). These results provide insight into the tertiary structure of human erythropoietin and suggest uses for the sequence-specific antibodies in labeling the hormone. |
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