| 人巨细胞病毒抗体检测方法的应用研究 |
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| 引用本文： | 彭良俊,周志军,李策生. 人巨细胞病毒抗体检测方法的应用研究[J]. 微生物学免疫学进展, 2014, 0(4): 11-15 |
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| 作者姓名： | 彭良俊 周志军 李策生 |
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| 作者单位： | 武汉生物制品研究所有限责任公司血液制剂研究室,湖北武汉430060 |
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| 基金项目： | 2013年湖北省重大科技创新计划（项目编号：2013ACC001） |
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| 摘 要： | 目的确定用于筛选人巨细胞病毒(HCMV)IgG阳性血浆的ELISA试剂和用于HCMV免疫球蛋白成品检定的中和试验方法。方法比较目前国内外现有的人巨细胞病毒IgG抗体检测方法(3家ELISA试剂和3种病毒中和试验)的相关性。结果德国赛润和意大利德塞的ELISA试剂与成都蓉生微量中和试验及台湾宝血的蚀斑减少中和试验的相关性很好(r299%)。结论从成本和使用便利性考虑,建议使用意大利德赛的ELISA试剂盒用于原料血浆的筛选,使用成都蓉生的微量中和试验作为成品效价检定的方法。
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| 关 键 词： | 巨细胞病毒抗体 ELISA 中和试验 相关性 |
Application in detection methods of human cytomegalovirus antibody |
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| PEN Liang-jun,ZHOU Zhi-jun,LI Ce-sheng. Application in detection methods of human cytomegalovirus antibody[J]. Progress In Microbiology and Immunology, 2014, 0(4): 11-15 |
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| Authors: | PEN Liang-jun ZHOU Zhi-jun LI Ce-sheng |
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| Affiliation: | (Wuhan Institute of Biological Products Co. ,Ltd. , Wuhan 430060, Hubei provincial, China) |
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| Abstract: | Objective To determine the ELISA kit of screening HCMV- IgG positive plasma and the method of neutraliza- tion test of the HCMV immunoglobulin in finished product. Methods Investigation was carried out in correlation of three current domestic and international existing ELISA kits in detection of human cytomegalovirus IgG antibody with virus neu- tralization tests developed by three company. Results ELISA kits from Germany SERION and Italy DIESSE have the good correlation with micro-scale neutralization test developed by Chengdu Rongsheng and plaque-reduction neutralization test by Taiwan Baoxue （r2〉99%）. Conclusions Regarding the cost and ease of use, it is recommended to use Italy DIESSE ELISA kit for material plasma screening, and to use Rongsheng micro-scale neutralization test as a method of testing the po- tency in finished product. |
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| Keywords: | Human cytomegalovirus IgG antibody ELISA Neutralization test Correlation |
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