无明胶布氏菌活疫苗冻干稳定剂的筛选

目的为皮上划痕人用布氏菌活疫苗筛选存活率高、无明胶冻干稳定剂。方法以冻干活菌存活率为指标,对甘油、甘露醇、蔗糖、葡萄糖、乳糖、谷氨酸钠、甘氨酸、谷氨酸、脯氨酸和硫脲等10种稳定剂通过单因素筛选法,筛选出冻干存活率高的4种单因素稳定剂成分;将4种单因素稳定剂成分进行正交试验优化,筛选出最优稳定剂组合。结果单因素试验结果显示,甘油、葡萄糖、谷氨酸钠和硫脲4种稳定剂成分冻干后活菌存活率较高,对布氏菌活疫苗具有良好保护效果。通过正交试验筛选出最优稳定剂配方中四组分的质量分数分别为甘油1.5%、葡萄糖5%、硫脲1.5%、谷氨酸钠1.0%,该配方的冻干存活率可达81.5%。结论无明胶冻干稳定剂对布氏菌活疫苗具有较好的保护作用。

Screening of a gelatin-free protective agent for freeze-dried live Brucella vaccine

Objective To screen a gelatin-free protective agent for freeze-dried live Brucella vaccine. Methods The protective effects of 10 different protective agents were compared with single factor such as glycerol,mannitol,sucrose,glucose,lactose,glutamine sodium,glycine,glutamic acid,proline and thiourea,and the orthogonal design was carried out. The viable count as a index was detected for selecting a better formula. Results The tested results showed that glycerol, glucose,glutamic sodium and thiourea is the good protective agent. The formula containing 1. 5% glycerol,5% glucose, 1. 5% thiourea and 1. 0% glutamic sodium is the most optimal formula. The viable rate of freeeze-dried vaccine after adding this formula agent could be achived 81. 5%. Conclusions The gelatin-free freeze-dried cryoprotectant is of the good protective effect on the live Brucella vaccine.