| 甲型副伤寒沙门菌培养条件的优化 |
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| 引用本文： | 陈国怀,曹玲,罗广,刘晓,刘大东,金红燕,陈刚,席仲兴.甲型副伤寒沙门菌培养条件的优化[J].微生物学免疫学进展,2014(4):26-28. |
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| 作者姓名： | 陈国怀 曹玲 罗广 刘晓 刘大东 金红燕 陈刚 席仲兴 |
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| 作者单位： | 兰州生物制品研究所有限责任公司甘肃省疫苗工程技术研究中心,甘肃兰州730046 |
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| 摘 要： | 优化甲型副伤寒沙门菌的培养条件,提高菌体产量。方法通过单因素及正交试验,对影响甲型副伤寒沙门菌生长的培养温度、NaCl浓度和pH等条件进行优化。结果甲型副伤寒沙门菌在NaCl浓度0.75%、温度35℃、pH6.5时菌体产量最高。结论通过对甲型副伤寒沙门菌培养条件进行优化,获得较高的菌体产量,为后期诊断试剂盒的开发奠定了基础。
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| 关 键 词： | 甲型副伤寒沙门菌 优化培养 |
Optimization on growth condition of Salmonella paratyphoid A |
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| CHEN Guo-huai,CAO Lin,LUO Guong,LIU Xiao,LIU DA-dong,JIN Hong-yan,XI Zhong-xing.Optimization on growth condition of Salmonella paratyphoid A[J].Progress In Microbiology and Immunology,2014(4):26-28. |
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| Authors: | CHEN Guo-huai CAO Lin LUO Guong LIU Xiao LIU DA-dong JIN Hong-yan XI Zhong-xing |
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| Institution: | (Lanzhou Institute of Biological Products Co. Ltd. , Center for Gansu Provincial Vaccine Engineering Rresearch, Lanzhou 730046, Gansu Provionce China) |
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| Abstract: | Objective To optimize the growth condition of Salmonella paratyphoid A. Methods To study the effect for temperature ,pH and NaCl concentration on the growth of Salmonella paratyphoid A by using the method of orthogonal design. Results The optimal culture condition of Salmonella paratyphoid A is NaCl concentration 0.75% , temperature 35 ℃ and pH 6.5. Conclusion The optimal culture condition of Salmonella paratyphoid A is successfully achieved in this investigation. |
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| Keywords: | Salmonella paratyphoid A Optimization for cuhivation |
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