Simultaneous determination of misonidazole and desmethylmisonidazole in plasma by high-performance liquid chromatography |
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Authors: | P. G. Meering R. A. A. Maes |
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Affiliation: | Centre for Human Toxicology, State University of Utrecht, Vondellaan 14, 3521 GE Utrecht The Netherlands |
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Abstract: | A high-performance liquid chromatographic method for the simultaneous determination of misonidazole and desmethylmisonidazole in plasma is described. After plasma is deproteinized with methanol and the diluted supernatant is chromatographed on a C18 reversed-phase column, both compounds are quantitated by means of an internal standard. The coefficients of variation of within-day and day-to-day precision are below 5.0% for misonidazole in the concentration range of 25–250 mg/l and below 6.1% for desmethylmisonidazole in the concentration range of 2.5–25.0 mg/l. Calibration curves are linear and an analytical recovery varying from 97.6 to 99.8% is obtained. The detection limits for misonidazole and desmethylmisonidazole in plasma are 1.4 mg/l and 0.7 mg/l, respectively. |
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