Purification and structure of the host-specific toxin from Helminthosporium carbonum race 1 |
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Authors: | J D Walton E D Earle B W Gibson |
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Affiliation: | Department of Plant Breeding and Biometry Cornell University Ithaca, New York 14853 USA;Department of Chemistry Massachusetts Institute of Technology Cambridge, Massachusetts 02139 USA |
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Abstract: | The host-specific toxin from Helminthosporiumcarbonum race 1 was purified from culture filtrates by solvent extraction, gel filtration, and high pressure liquid chromatography. High resolution mass spectrometry of the purified toxin gave a MW of 436.2318 and an elemental composition C21H32N4O6. Amino acid analysis and proton and13C-NMR indicated a peptide containing four amino acids. Their sequence was determined by gas chromatography mass spectrometry. Finally, digestion of the amino acids with D- and L-amino acid oxidases gave the complete structure cyclo[(L-2-amino-9, 10-epoxy-8-oxodecanoyl)-D-prolyl-L-alanyl-L-alanyl]. |
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