Berberine synthesis by callus and cell suspension cultures of Coscinium fenestratum |
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Authors: | A Jayakumaran Nair P R Sudhakaran J Madhusudana Rao S V Ramakrishna |
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Institution: | (1) Regional Research Laboratory (CSIR), 695019 Trivandrum, India;(2) Department of Biochemistry, University of Kerala, 695017 Trivandrum, India |
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Abstract: | Callus and cell suspension cultures of Coscinium fenestratum were established from sterile petiole segments on Murashige & Skoog (MS) medium, supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) and benzyl amino purine (BAP). The cells in the culture produced berberine as the major compound. NAA stimulated the product synthesis over 2,4-D. Presence of light inhibited the growth and enhanced the berberine synthesis.Abbreviations BAP
6-benzylaminopurine
- 2,4-D
2,4-dichlorophenoxy acetic acid
- HPLC
high pressure liquid chromatography
- IAA
indole-3-acetic acid
- NAA
-naphthaleneacetic acid
- TLC
thin layer chromatography |
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Keywords: | berberine callus cell culture Coscinium fenestratum Menispermaceae suspension |
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