Vacuolar protein sorting: two different functional states of the AAA-ATPase Vps4p |
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Authors: | Hartmann Claudia Chami Mohamed Zachariae Ulrich de Groot Bert L Engel Andreas Grütter Markus G |
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Affiliation: | 1 Institute of Biochemistry, University of Zürich, Winterthurer Strasse 190, 8057 Zürich, Switzerland 2 M.E. Institute for Structural Biology, Biozentrum, University of Basel, 4056 Basel, Switzerland 3 Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
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Abstract: | The vacuolar protein sorting (Vps) pathway, in which Vps4 class I AAA-ATPases play a central role, regulates growth factor receptors, immune response, and developmental signaling, and participates in tumor suppression, apoptosis, and retrovirus budding. We present the first atomic structure of the nucleotide-free yeast His6ΔNVps4p dimer and its AMPPNP (5′-adenylyl-β,γ-imidodiphosphate)-bound tetradecamer, derived from a cryo electron microscopy map. Vps4p dimers form two distinct heptameric rings and accommodate AAA cassettes in a head-to-head—not in a head-to-tail—fashion as in class II AAA-ATPases. Our model suggests a mechanism for disassembling ESCRT (endosomal sorting complex required for transport) complexes by movements of substrate-binding domains located at the periphery of the tetradecamer during ATP hydrolysis in one ring, followed by translocation through the central pore and ATP hydrolysis in the second ring. |
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Keywords: | Vps, vacuolar protein sorting AMPPNP, 5&prime -adenylyl-β,γ-imidodiphosphate EM, electron microscopy MD, molecular dynamics EDTA, ethylenediaminetetraacetic acid |
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