| 老龙皮总氨基酸的提取工艺研究 |
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| 引用本文： | 王晓梅,赵卫星,常军林,刘莎,房咪. 老龙皮总氨基酸的提取工艺研究[J]. 中国野生植物资源, 2010, 29(6): 51-53. DOI: 10.3969/j.issn.1006-9690.2010.06.014 |
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| 作者姓名： | 王晓梅 赵卫星 常军林 刘莎 房咪 |
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| 作者单位： | [1]宝鸡文理学院化学化工系,陕西宝鸡721013 [2]陕西省植物化学重点实验室,陕西宝鸡721013 [3]宝鸡市中医医院,陕西宝鸡721004 |
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| 基金项目： | 陕西省教育厅重点实验室基金资助项目，宝鸡文理学院院级重点项目 |
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| 摘 要： | 通过单因素选择和正交设计法,以老龙皮总氨基酸含量为指标,分别对乙醇浓度、料液比、提取温度、提取时间等因素进行考查,优选老龙皮总氨基酸的最佳提取条件。结果显示料液比对总氨基酸提取率影响最大,最佳提取条件为60%乙醇作提取液,料液比(W/V)为1∶20,在70℃提取3 h。
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| 关 键 词： | 老龙皮 氨基酸 正交试验 提取工艺 |
Study on the Extraction Process of Total Amino Acids from Lobaria kurokavae Yoshim |
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| Wang Xiaomei,Zhao Weixing,Chang Junlin,Liu Sha,Fang Mi. Study on the Extraction Process of Total Amino Acids from Lobaria kurokavae Yoshim[J]. Chinese Wild Plant Resources, 2010, 29(6): 51-53. DOI: 10.3969/j.issn.1006-9690.2010.06.014 |
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| Authors: | Wang Xiaomei Zhao Weixing Chang Junlin Liu Sha Fang Mi |
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| Affiliation: | 1.Department of Chemistry and Chemical Engineering,Baoji University of Arts and Sciences,Baoji 721013,China;2.Shannxi Key Laboratory of Phytochemistry,Baoji 721013,China;3.Baoji Hospital of Traditional Chinese Medicine,Baoji 721004,China) |
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| Abstract: | Adopting single factor trial,the orthogonal test was designed to optimizate the extraction conditions of total amino acids from Lobaria kurokavae Yoshim.According to the output of total amino acids under the different extraction conditions,which were selected as solvent concentration,ratio of liquid to material,extraction temperature and extraction time for extraction process.Results showed that the ratio of liquid to material was the main factor on the extraction process.The optimum conditions were established as follows： the concentration of alcohol was 60%,volume of alcohol was 20 times amount of herb,and the herb was extracted 3hours with temperature 70℃. |
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| Keywords: | Lobaria kurokavae Yoshim amino acids orthogonal test extraction process |
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