Introduction of customized inserts for streamlined assembly and optimization of BioBrick synthetic genetic circuits |
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| Authors: | Julie E Norville,Ratmir Derda,Saurabh Gupta,Kelly A Drinkwater,Angela M Belcher,Andres E Leschziner,Thomas F Knight Suffix" >Jr |
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| Affiliation: | 1.Computer Science and Artificial Intelligence Laboratory,Massachusetts Institute of Technology,Cambridge,USA;2.Biological Engineering Division,Massachusetts Institute of Technology,Cambridge,USA;3.Department of Materials Science and Engineering,Massachusetts Institute of Technology,Cambridge,USA;4.Department of Chemistry,University of Alberta,Edmonton,Canada;5.Department of Chemistry and Chemical Biology,Harvard University,Cambridge,USA;6.Wyss Institute for Biologically Inspired Engineering,Harvard University,Cambridge,USA;7.Department of Molecular and Cellular Biology,Harvard University,Cambridge,USA;8.Unit 2B,Ginkgo BioWorks,Boston,USA |
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| Abstract: | Background BioBrick standard biological parts are designed to make biological systems easier to engineer (e.g. assemble, manipulate, and modify). There are over 5,000 parts available in the Registry of Standard Biological Parts that can be easily assembled into genetic circuits using a standard assembly technique. The standardization of the assembly technique has allowed for wide distribution to a large number of users -- the parts are reusable and interchangeable during the assembly process. The standard assembly process, however, has some limitations. In particular it does not allow for modification of already assembled biological circuits, addition of protein tags to pre-existing BioBrick parts, or addition of non-BioBrick parts to assemblies. |
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