Improved somatic embryo maturation in loblolly pine by monitoring ABA-responsive gene expression |
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| Authors: | Teresa Vales Xiaorong Feng Lin Ge Nanfei Xu John Cairney Gerald S Pullman Gary F Peter |
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| Institution: | (1) Institute of Paper Science and Technology, 500 10th Street, NW Atlanta, GA 30318-5794, USA;(2) Present address: Arborgen, P.O. Box 840001, Summerville, SC 29484, USA;(3) Present address: Argos Therapeutics, 4233 Technology Drive, Durham, NC 27704, USA;(4) Present address: BASF Plant Science, NC, 26 Davis Drive, RTP, NC 27709, USA;(5) Present address: School of Forest Resources and Conservation, University of Florida, P.O. Box 110410, Gainesville, FL 32611-0410, USA |
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| Abstract: | During loblolly pine zygotic embryo development, increases in mRNAs for three ABA-responsive LEA-like genes coincided with
the two developmental stage-specific peaks of endogenous ABA accumulation (Kapik et al. 1995). These ABA concentration profiles from zygotic embryo development were used to develop several tissue culture approaches
that altered the exposure of somatic embryos to exogenous ABA. Elevating exogenous ABA at a time corresponding to mid-maturation
improved the germination and resulted in more zygotic-like expression of selected genes in somatic embryos. Extending the
time on maturation medium for a fourth month increased embryo yield, dry weight, and germination in high-and low-yield genotypes.
Optimizing the amounts of embryogenic suspension, plated and exogenous ABA concentration increased from 22 to 66% in the early-stage
bipolar embryos that developed to the cotyledonary stage. |
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| Keywords: | Somatic embryogenesis Cell density Abscisic acid Loblolly pine Gene expression |
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