Attachment of a fluorescent label to 4-thiouracil and 4-thiouridine |
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| Authors: | J A Secrist J R Barrio N J Leonard |
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| Affiliation: | 1. Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada;2. Department of Immunology, University of Manitoba, Winnipeg, MB, R3E 0T5, Canada;3. Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada;4. Biotechnology Institute Thurgau (BITg), University of Konstanz, Unterseestrasse 47, CH-8280 Kreuzlingen, Switzerland;1. Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan;2. Division of Biology & Geosciences, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan;3. Department of Biochemistry, Faculty of Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan |
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| Abstract: | The conversion of 4-thiouracil and 4-thiouridine into fluorescent derivatives has been carried out using 4-bromomethyl-7-methoxy-2-oxo-2H-benzopyran. Adsorption of the water-insoluble reagent onto Celite allowed the reaction to be applied to completely aqueous media. The conditions employed for the reaction, pH 8.3–8.4 and 37°, are such as to allow ready extension to a tRNA. The blue-white fluorescence of the product (λmax = 400 nm) is visible down to concentrations on the order of 10−7M. |
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