Influence of jasmonic acid as potential activator of induced resistance against Karnal bunt in developing spikes of wheat |
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Authors: | Mihir K Mandal Dinesh Pandey Shalini Purwar U S Singh Anil Kumar |
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Institution: | (1) Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, 263 145 Pantnagar, India;(2) Department of Plant Pathology, College of Agriculture GB Pant University of Agriculture and Technology, 263 145 Pantnagar, India |
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Abstract: | Induction of defense response against Karnal bunt (KB) by suppressing the pathogenesis was observed upon exogenous application
of jasmonic acid (JA) as evident from decrease in the coefficient of infection and overall response value in both susceptible
and resistant varieties of wheat. The ultra-structural changes during disease progression showed the signs of programmed cell
death (PCD). However, JA strengthened the defense barrier by enhancing the lignifications of cell walls as observed in spikes
of both varieties by histochemical analysis. Compared to the plants inoculated with pathogen alone, plants of resistant line
(RJP) first treated with JA followed by inoculation with pathogen showed more lignifications and extracellular deposition
of other metabolites on cells, which is supposed to prevent mycelial invasions. Contrary to this, susceptible (SJP) lines
also showed lignifications but the invasion was more compared to resistant line. Induction of protease activity was higher
in resistant variety than its corresponding susceptible variety. The protease activity induced during the colonization of
the pathogen and its proliferation inside the host system gets inhibited by JA treatment as demonstrated by the quantitative
and in-gel protease assay. The results indicate the role of JA signalling in inhibiting the proteases due to expression of
certain protease inhibitor genes. SDS-PAGE analysis shows differential gene expression through induction and/or suppression
of different proteins in wheat spikes of resistant and susceptible varieties under the influence of JA. Thus, exogenously
applied JA provides the conditioning effect prior to the challenge of infection and induces defense against KB probably by
maintaining a critical balance between proteases and protease inhibitors and/or coordinating induction of different families
of new proteins. |
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Keywords: | Jasmonic acid Karnal bunt induced resistance protease protease inhibitor |
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