DNA degradation in avian faecal samples and feasibility of non-invasive
genetic studies of threatened capercaillie populations |
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Authors: | Sébastien Regnaut Françoise S Lucas Luca Fumagalli |
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Institution: | (1) Department of Ecology and Evolution, Laboratory for Conservation Biology, University of Lausanne, CH-1015 Lausanne, Switzerland;(2) EAWAG, Limnological Research Center, CH-6047 Kastanienbaum, Switzerland |
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Abstract: | We evaluated the feasibility of using faeces as a non-invasively collected DNA source for the genetic study of an endangered bird population (capercaillie; Tetrao urogallus). We used a multitube approach, and for our panel of 11 microsatellites genotyping reliability was estimated at 98% with five repetitions. Experiments showed that free DNases in faecal material were the major cause of DNA degradation. Our results demonstrate that using avian faeces as a source of DNA, reliable microsatellite genotyping can be obtained with a reasonable number of PCR replicates. |
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Keywords: | bird DNA degradation faeces genotyping errors microsatellites non-invasive sampling |
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