Potential of a granulovirus isolate to control <Emphasis Type="Italic">Phthorimaea operculella</Emphasis> (Lepidoptera: Gelechiidae) |
| |
Authors: | Gabriel Moura Mascarin Sérgio Batista Alves Fátima Teresinha Rampelotti-Ferreira Mariana Ragassi Urbano Clarice Garcia Borges Demétrio Jr" target="_blank">Italo DelaliberaJr |
| |
Institution: | 1.Departamento de Entomologia e Acarologia,Escola Superior de Agricultura “Luiz de Queiroz”, ESALQ/USP,Piracicaba,Brazil;2.Exact Sciences Department,ESALQ/University of S?o Paulo,Piracicaba,Brazil |
| |
Abstract: | In this study a Brazilian granulovirus strain, PhopGV, isolated from the potato tuber moth (PTM) Phthorimaea operculella, was investigated regarding its potential for biological control and in vivo production. The relationship between mortality
of P. operculella larvae and virus concentration was determined at different temperatures on potato tubers and susceptibility of P. operculella to PhopGV was also determined on potato leaves. Virulence of PhopGV to P. operculella was not affected by temperatures from 18 to 30°C. The median lethal concentration (LC50) of larvae fed on potato foliage treated with PhopGV was not higher than that verified with larvae fed on treated tubers.
Optimal conditions for production of virus-infected larvae were obtained by using the virus suspensions of 41 × 105, 6.3 × 105 and 62 × 105 OBs ml−1 at 18, 24 and 30°C, which resulted in 32.0, 31.4 and 34.8% of infected larvae collected, respectively. The maximum percentage
of infected larvae recovered from tubers was not affected by temperature. However, time for production of virus-infected larvae
was longer at 18°C and shorter at 30°C. Persistence of PhopGV was determined on stored tubers and we observed that the virus
remained effective for at least two months, causing up to 84.2% mortality of P. operculella at 1 × 107 OBs ml−1. The pathogen was also highly virulent to tomato pinworm, Tuta absoluta, inflicting high percentage of mortality, delaying larval growth and inhibiting pupation. This Brazilian PhopGV strain has
potential to control PTM larvae on potato tubers at a broad range of temperature and can be produced in vivo using virus-treated
tubers. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|