| 灵芝漆酶催化直接耐晒翠蓝GL脱色条件的优化 |
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| 引用本文： | 陈琼华,周玉萍,杨桃芳,程惠贞,田长恩.灵芝漆酶催化直接耐晒翠蓝GL脱色条件的优化[J].微生物学通报,2009,36(12):1812-1817. |
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| 作者姓名： | 陈琼华 周玉萍 杨桃芳 程惠贞 田长恩 |
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| 作者单位： | 广州大学生命科学学院 广东 广州 510006;广州大学植物抗逆基因功能研究广州市重点实验室 广东 广州 510006;广州大学生命科学学院 广东 广州 510006;广州大学植物抗逆基因功能研究广州市重点实验室 广东 广州 510006;广州大学生命科学学院 广东 广州 510006;广州大学植物抗逆基因功能研究广州市重点实验室 广东 广州 510006 |
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| 基金项目： | 广东省自然科学基金项目(No. 8151009101000013); 广东省科技计划项目(No. 2007B080701008); 广州市高校科学技术研究项目(No. 62031); 广州大学植物抗逆基因功能研究广州市重点实验室开放基金项目(No. 200801) |
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| 摘 要： | 采用灵芝菌株发酵所得的漆酶, 对酞菁类染料直接耐晒翠蓝GL进行了催化脱色实验, 确定了脱色反应的最适条件。结果表明: 单独使用灵芝漆酶粗酶液对直接耐晒翠蓝GL具有很好的脱色效果。其最适脱色pH为3.0, 最适脱色温度为40°C, 最适漆酶用量是40 U/mL, 最适染料浓度为60 mg/L。以上述最适脱色条件对直接耐晒翠蓝GL进行脱色实验, 反应70 min, 脱色率可达94.3%。研究结果显示, 所试灵芝漆酶在印染废水治理方面具有良好的应用前景。
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| 关 键 词： | 漆酶 直接耐晒翠蓝GL 脱色 灵芝 |
Optimization of Conditions in Decolorization of Direct Fast Turquoise Blue GL Catalyzed by Laccase from Ganoderma lucidum |
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| CHEN Qiong-Hu,ZHOU Yu-Ping,YANG Tao-Fang,CHENG Hui-Zhen and TIAN Chang-En.Optimization of Conditions in Decolorization of Direct Fast Turquoise Blue GL Catalyzed by Laccase from Ganoderma lucidum[J].Microbiology,2009,36(12):1812-1817. |
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| Authors: | CHEN Qiong-Hu ZHOU Yu-Ping YANG Tao-Fang CHENG Hui-Zhen and TIAN Chang-En |
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| Institution: | School of Life Sciences, Guangzhou University, Guangzhou, Guangdong 510006, China;Guangzhou Key Laboratory for Functional Studies on Plant Stress-Resistant Genes, Guangzhou University, Guangzhou, Guangdong 510006, China;School of Life Sciences, Guangzhou University, Guangzhou, Guangdong 510006, China;Guangzhou Key Laboratory for Functional Studies on Plant Stress-Resistant Genes, Guangzhou University, Guangzhou, Guangdong 510006, China;1. School of Life Sciences, Guangzhou University, Guangzhou, Guangdong 510006, China; 2. Guangzhou Key Laboratory for Functional Studies on Plant Stress-Resistant Genes, Guangzhou University, Guangzhou, Guangdong 510006, China |
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| Abstract: | The decolorization experiments of direct fast turquoise blue GL were conducted with the crude laccase from Ganoderma lucidum in submerged fermentation. The optimum decolorization conditions were determined. The results showed that the crude laccase from Ganoderma lucidum had distinct decolorization efficiency on direct fast turquoise blue GL. The optimum pH was 3.0, The optimum temperature was 40°C, The optimum enzyme dosage was 40 U/mL, the optimum dye concentrations was 60 mg/L. Under the best conditions optimized above, the decolorization rate of direct fast turquoise blue GL was 94.3% after 70 min catalyzation with the laccase from G. lucidum. Our results suggested that the laccase from Ganoderma lucidum could be very useful in the textile-dye decolorization and waste-water purification. |
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| Keywords: | Laccase Direct fast turquoise blue GL Decolorization Ganoderma lucidum |
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