Specific discrimination of chicken DNA from other poultry DNA in processed foods using the polymerase chain reaction |
| |
Authors: | Fujimura Tatsuya Matsumoto Takashi Tanabe Soichi Morimatsu Fumiki |
| |
Institution: | Research and Development Center, Nippon Meat Packers, Inc., Midorigahara, Tsukuba, Ibaraki 300-2646, Japan. t.fujimura@nipponham.co.jp |
| |
Abstract: | In the present study, specific discrimination of chicken DNA contamination in processed foods using the polymerase chain reaction was investigated. The primer pair was designed to amplify a 102-bp fragment of the chicken mitochondrial 16S ribosomal RNA gene. While the DNA from chicken meat was amplified, the DNA from other poultry meat, mammalian meat, fish, shellfish, and cereals was not amplified. The primer amplified DNA fragments derived from model processed and nonprocessed food samples containing 0.001, 0.01, 0.1, 1, 10, and 100% chicken. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|