| 铕标记抗癌胚抗原单克隆抗体C17的研究和应用 |
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| 引用本文： | 赵启仁,张福华,刘洁,林汉,李美佳,庄湘莲,陈艾,胡璧.铕标记抗癌胚抗原单克隆抗体C17的研究和应用[J].生物化学与生物物理进展,1995,22(1):43-47. |
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| 作者姓名： | 赵启仁 张福华 刘洁 林汉 李美佳 庄湘莲 陈艾 胡璧 |
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| 作者单位： | 中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所;中国医学科学院放射医学研究所 |
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| 基金项目： | 国家自然科学基金资助. |
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| 摘 要： | 用合成的N′-(对-异硫氰基苄基)-二乙三胺-N1,N2,N3,N3-四乙酸铕钠为螫合剂.用Sephadex G-50凝胶柱层析分离标记C17和过量试剂.分部收集的层析物的光密度图和荧光强度图完全一致.蛋白峰处两管标记C17的比活性分别为5.2和8.9Eu3+离子每C17分子,而且C17的标记回收率为64%.标记C17至少在6个月内是稳定的.用铕标记物得到的时间分辨免疫荧光分析的标准曲线具有良好线性和斜率,表明标记物免疫活性良好,而且已用于血清CEA分析.
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| 关 键 词： | 时间分辨荧光免疫分析 时间分辨免疫荧光分析 非同位素免疫分析 铕标记 |
| 收稿时间： | 1993/12/20 0:00:00 |
| 修稿时间： | 1994/3/29 0:00:00 |
Study and Application of Europium-Labeled McAb C17 Against CEA |
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| Zhao Qiren,Zhang Fuhu,Liu Jie,Lin Han,Li Meiji,Zhuang Xianglian,Chen Ai and Hu Bi.Study and Application of Europium-Labeled McAb C17 Against CEA[J].Progress In Biochemistry and Biophysics,1995,22(1):43-47. |
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| Authors: | Zhao Qiren Zhang Fuhu Liu Jie Lin Han Li Meiji Zhuang Xianglian Chen Ai and Hu Bi |
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| Institution: | Institute of Radiation Medicine, Chinese Academy of Medical Sciences;Institute of Radiation Medicine, Chinese Academy of Medical Sciences;Institute of Radiation Medicine, Chinese Academy of Medical Sciences;Institute of Radiation Medicine, Chinese Academy of Medical Sciences;Institute of Radiation Medicine, Chinese Academy of Medical Sciences;Institute of Radiation Medicine, Chinese Academy of Medical Sciences;Institute of Radiation Medicine, Chinese Academy of Medical Sciences;Institute of Radiation Medicine, Chinese Academy of Medical Sciences |
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| Abstract: | Study and application of europium (Eu) labeled mouse monoclonal antibody (McAb) C17 against carcinoembryonic antigen (CEA) by using a synthesized chelate-N1-(p-isothiocyanatobenzyl)-diethylenetriamine-N1,N2,N3, N3-tetraacetic acid Eu is described.The Eu-labeled C17 was separated from excess reagent by gel filtration on a column of Sephadex G-50.The fluorescence and ultraviolet absorption for the fractions were monitored and the curves of fluorescence and the optical density were all the same.The specific activity for the two tubes of the labeled-C17 at the protein peak was 5.2 and 9.8 Eu3+/C17 molecule respectively and the recovery of the labeled-C17 was 64%. The labeled-C17 was stable for 6 months at least. The standard curves of time-resolved immunofluorometric assay using the labled-C17 showed excellent linearity and slope and had been used in the assay of CEA in serum. |
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| Keywords: | time-resolved fluoroimmunoassay time-resolved immunofluorometricy assay nonisotopic immunoassay europium label |
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