| 氮饥饿补糖分批培养小克银汉霉产γ-亚麻酸的研究 |
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| 引用本文： | 曾杨,余龙江,朱敏,周蓬蓬,何贶.氮饥饿补糖分批培养小克银汉霉产γ-亚麻酸的研究[J].生命科学研究,2004,8(1):86-88. |
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| 作者姓名： | 曾杨 余龙江 朱敏 周蓬蓬 何贶 |
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| 作者单位： | 华中科技大学,生命科学与技术学院,中国,湖北,武汉,430074 |
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| 摘 要： | 对小克银汉霉C2(Cunninghamella sp．C2)发酵生产γ-亚麻酸工艺进行研究，发现氮饥饿补糖能有效地积累γ-亚麻酸，在第5、6、7d每天补糖15g／L，培养10d后生物量、油脂和γ-亚麻酸的产量分别达到47．4g／L、19.74g／L和1．86g／L，为分批培养的2．85、2．08和2．07倍。
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| 关 键 词： | 小克银汉霉 γ-亚麻酸 生物量 油脂 培养 氮饥饿补糖 微生物发酵法 GLA |
| 文章编号： | 1007-7847(2004)01-0086-03 |
| 修稿时间： | 2003年10月14 |
Study on Fed-batch Culture of Strain Cunninghamella sp.to Produce Gamma-linolenic Acid by Nitrogen Starvation and Glucose Feeding |
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| ZENG Yang,YU Long-Jiang,ZHU Min,ZHOU Peng-peng,HE Kuang.Study on Fed-batch Culture of Strain Cunninghamella sp.to Produce Gamma-linolenic Acid by Nitrogen Starvation and Glucose Feeding[J].Life Science Research,2004,8(1):86-88. |
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| Authors: | ZENG Yang YU Long-Jiang ZHU Min ZHOU Peng-peng HE Kuang |
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| Abstract: | Fermentation strategies for producing gamma-linolenic acid by Cunninghamella C2 were studied. During the cultivation, the method of nitrogen starvation and glucose feeding to accumulate gamma-linolenic acid were investigated. 15 g/L concentration of glucose were fed at the fifth, sixth and seventh day. After 10 days of cultivation, the yield of biomass, total lipid and total GLA were 47. 4 g/L, 19. 74 g/L and 1. 86 g/L, respectively, which were 2. 85, 2. 08 and 2. 07 folds of those of the control. |
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| Keywords: | nitrogen starvation and glucose feeding Cunninghamella sp gamma-linolenic acid |
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