ATP-Citrate Lyase and Other Enzymes of Acetyl-CoA Metabolism in Fractions of Small and Large Synaptosomes from Rat Brain Hippocampus and Cerebellum |
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| Authors: | Andrzej Szutowicz ‡ Norman F Harris †¶ Paul A Srere ‡ Isaac L Crawford†¶§ |
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| Institution: | Preclinical Science Unit, Veterans Administration Medical Center, The University of Texas Health Science Center, Dallas, Texas, U.S.A.;Laboratory of Neurophysiology, Veterans Administration Medical Center, The University of Texas Health Science Center, Dallas, Texas, U.S.A.;Department of Biochemistry, The University of Texas Health Science Center, Dallas, Texas, U.S.A.;Department of Neurology, The University of Texas Health Science Center, Dallas, Texas, U.S.A.;Department of Pharmacology, The University of Texas Health Science Center, Dallas, Texas, U.S.A. |
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| Abstract: | The activities of choline acetyltransferase and ATP-citrate lyase were significantly correlated (r = 0.995) in fractions of small and large synaptosomes isolated from rat hippocampus and cerebellum. The activities of these two enzymes did not correlate with those of pyruvate dehydrogenase, carnitine acetyltransferase, citrate synthase, acetyl-CoA synthetase, lactate dehydrogenase, or with the rate of high-affinity glutamate uptake in the synaptosomal fractions. The results provide additional evidence linking ATP-citrate lyase to the cholinergic system in the brain. |
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| Keywords: | ATP-citrate lyase Cholinergic system Large and small synaptosomes |
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