The Crystal Structure of Ferritin from Helicobacter pylori Reveals Unusual Conformational Changes for Iron Uptake |
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Authors: | Ki Joon Cho Hye Jeong Shin Ji-Hye Lee Sarah S. Park Cheolju Lee Kyung Hyun Kim |
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Affiliation: | 1 Department of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea 2 Department of Biomicrosystem Technology, Korea University, Seoul 136-701, Korea 3 Department of Food Technology, Korea University, Seoul 136-701, Korea 4 Pohang Accelerator Laboratory, Pohang University of Science and Technology, Pohang, Kyungbuk 790-784, Korea 5 Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 120-750, Korea 6 Life Sciences Division, Korea Institute of Science and Technology, Seoul 136-791, Korea 7 Department of Biotechnology and Bioinformatics, Korea University, Chungnam 339-700, Korea |
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Abstract: | The crystal structure of recombinant ferritin from Helicobacter pylori has been determined in its apo, low-iron-bound, intermediate, and high-iron-bound states. Similar to other members of the ferritin family, the bacterial ferritin assembles as a spherical protein shell of 24 subunits, each of which folds into a four-α-helix bundle. Significant conformational changes were observed at the BC loop and the entrance of the 4-fold symmetry channel in the intermediate and high-iron-bound states, whereas no change was found in the apo and low-iron-bound states. The imidazole rings of His149 at the channel entrance undergo conformational changes that bear resemblance to heme configuration and are directly coupled to axial translocation of Fe ions through the 4-fold channel. Our results provide the first structural evidence of the translocation of Fe ions through the 4-fold channel in prokaryotes and the transition from a protein-dominated process to a mineral-surface-dominated process during biomineralization. |
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Keywords: | ROS, reactive oxygen species Bfr, bacterioferritin Hpf, Helicobacter pylori ferritin Cjf, Campylobacter jejuni ferritin Ecf, Escherichia coli ferritin f, human H ferritin HuLf, human L ferritin HuMf, human M ferritin NAP, neutrophil-activating protein ICP, inductively coupled plasma |
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