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Two Structurally Independent Domains of E. coli NusG Create Regulatory Plasticity via Distinct Interactions with RNA Polymerase and Regulators
Authors:Rachel Anne Mooney,Paul Rö  sch,Robert Landick
Affiliation:1 Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
2 Department of Biopolymers, Universitaet Bayreuth, 95440 Bayreuth, Germany
3 Department of Biochemistry and Molecular Biophysics and Department of Microbiology, Columbia University Medical Center, New York, NY 10032, USA
4 Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA
Abstract:
Keywords:EC, elongation complex   NTD, N-terminal domain   CTD, C-terminal domain   RNAP, RNA polymerase   EcNusG, Escherichia coli NusG   NGN, NusG N-terminal   TtNusG, Thermus thermophilus NusG   AaNusG, Aquifex aeolicus NusG   DSIF, DRB sensitivity-inducing factor   NELF, negative elongation factor   FL, full-length   HSQC, heteronuclear single quantum coherence   PDB, Protein Data Bank   NOESY, nuclear Overhauser enhancement spectroscopy   OD600, optical density at 600   nm
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