Two Structurally Independent Domains of E. coli NusG Create Regulatory Plasticity via Distinct Interactions with RNA Polymerase and Regulators |
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Authors: | Rachel Anne Mooney,Paul Rö sch,Robert Landick |
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Affiliation: | 1 Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA 2 Department of Biopolymers, Universitaet Bayreuth, 95440 Bayreuth, Germany 3 Department of Biochemistry and Molecular Biophysics and Department of Microbiology, Columbia University Medical Center, New York, NY 10032, USA 4 Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA |
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Abstract: | |
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Keywords: | EC, elongation complex NTD, N-terminal domain CTD, C-terminal domain RNAP, RNA polymerase EcNusG, Escherichia coli NusG NGN, NusG N-terminal TtNusG, Thermus thermophilus NusG AaNusG, Aquifex aeolicus NusG DSIF, DRB sensitivity-inducing factor NELF, negative elongation factor FL, full-length HSQC, heteronuclear single quantum coherence PDB, Protein Data Bank NOESY, nuclear Overhauser enhancement spectroscopy OD600, optical density at 600  nm |
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