Production of proteinase and phospholipase by Paracoccidioides brasiliensis |
| |
Authors: | de Assis C M Gambale W Paula C R |
| |
Institution: | 1.Se??o de Bioquímica, Divis?o de Patologia, Instituto Adolfo Lutz, S?o Paulo, Brasil ;2.Departamento de Microbiologia, Instituto de Ciencias Biomédicas, Universidade de S?o Paulo, S?o Paulo, Brasil ; |
| |
Abstract: | We have investigated the production of proteinase and phospholipase by 20 different isolates of Paracoccidioides brasiliensis. Isolates were grown in Bacto-peptone, Dextrose, pH 5.5, agar slants, at 27 °C for 30 days, and cultures were transferred
onto Petri dishes containing basis medium and bovine serum albumin fraction V and sterile egg yolk as substrates for enzyme
production, and incubated at 27 °C. After 30 days net enzyme activity was visualized and quantitavely evaluated, measuring
a ratio between colony diameter and diameter of the transparent (proteinase) or white (phospholipase) ring zone surrounding
it. Results demonstrated that all isolates had the ability to produce proteinase and phospholipase, even though variability
in enzyme production was noted among different isolates of P. brasiliensis.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | enzymes fungi hyphomycetes Paracoccidioides phospholipase proteinase |
本文献已被 PubMed SpringerLink 等数据库收录! |
|