Effect of the amino acid substitution in the DNA-binding domain of the Fur regulator on production of pyoverdine |
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Authors: | Renáta Vale?ová Andrea Palyzová Helena Mare?ová Václav ?těpánek Peter Babiak Pavel Kyslík |
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Institution: | 1. Laboratory of Enzyme Technology, Institute of Microbiology v.v.i, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague, Czech Republic
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Abstract: | The ferric uptake regulator gene (fur), its promoter region and Fur box of pvdS gene involved in siderophore-mediated iron uptake system were sequenced in the parent strain Pseudomonas aeruginosa PAO1 and in the fur mutant FPA121 derived from the strain PAO1. We identified the gene fur 179 bearing a novel, single-point mutation that changed the amino acid residue Gln60Pro in the DNA-binding domain of the Fur protein. The synthesis of pyoverdine was studied in cultures of the strains PAO1 and FPA121 grown in iron-deplete and iron-replete (60 μmol/L FeIII) medium. The amino acid replacement in the regulatory Fur protein is responsible for the overproduction of pyoverdine in iron-deplete and iron-replete medium. No mutation was identified in the Fur box of the gene pvdS. |
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