The lamin Dm0 allele Ari3 acts as an enhancer of position effect variegation of the wm4 allele in Drosophila |
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Authors: | Bao Xiaomin Girton Jack Johansen Jørgen Johansen Kristen M |
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Institution: | (1) Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 3154 Molecular Biology Building, Ames, Iowa 50011, USA |
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Abstract: | The association of lamin and lamin binding proteins with peripheral heterochromatin suggests the possibility that lamins may
influence gene expression by participating in the epigenetic regulation of chromatin stucture. To test this hypothesis we
have examined the effect of a recently generated partial loss-of-function lamin Dm0 allele Ari3 on PEV of the w
m4
allele in the Drosophila eye. The Lam
Ari3
allele is characterized by a truncation of the COOH-terminal domain and lacks the CaaX box that localizes lamin to the inner
nuclear membrane. We show that the Lam
Ari3
allele strongly increased silencing of w
m4
expression, thus acting as an enhancer of PEV. These results indicate that lamins may be involved in regulating gene silencing
and heterochromatic spreading at the w
m4
locus and provide evidence that lamins may contribute to the regulation of higher-order chromatin organization. |
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Keywords: | Lamin Dm0 Eye w m4 inversion Position effect variegation Drosophila |
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