Germinal centres and the B-cell system. V |
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Authors: | Nicolette A Gastkemper Auk S Wubbena Felix J H Gimbrère Alexander de Graaff Paul Nieuwenhuis |
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Institution: | (1) Department of Histology, University of Groningen, Groningen, The Netherlands;(2) Department of Histology, University of Groningen, Oostersingel 69/1, 9713 EZ Groningen, The Netherlands |
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Abstract: | Summary Thoracic duct lymphocytes (TDL) were studied with respect to their capacity to give rise to germinal centres (GC) and to form primary antibody in an adoptive transfer system of the rat. Challenge with sheep erythrocytes (SRBC) 24h after lethal irradiation (900 rads) and syngeneic TDL reconstitution (108) lead to conspicuous GC activity already 7 days after transfer. In contrast, using syngeneic bone marrow (BM) in the adoptive transfer system, no GC formation was observed over the period studied (14 days after reconstitution). Reconstitution experiments using in vivo-separated T-TDL (1–5 % s-Ig+) and B-TDL (>90% s-Ig+) subpopulations, either separately or in combination, indicated that GC originate from B-TDL but require T-TDL for induction.Abbreviations BM
Bone Marrow
- TDL
Thoracic Duct Lymphocytes
- GC
Germinal Centre
- SRBC
Sheep Red Blood Cells
- GCDC
Germinal Centre-Derived Cells
- GCPC
Germinal Centre-Precursor Cells
- DAB
Mineral Salt Solution Dulbecco A + B
- FCS
Fetal Calf Serum
- PALS
Peri-Arteriolar Lymphocyte Sheath
- AFCP
Antibody-Forming Cell Precursors |
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Keywords: | Thoracic duct-lymphocytes B-cell differentiation Germinal centre Germinal centre-precursor cell Antibody-forming cell precursor |
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