Production of antioxidant and antitumor metabolites by submerged cultures of <Emphasis Type="Italic">Inonotus obliquus</Emphasis> cocultured with <Emphasis Type="Italic">Phellinus punctatus</Emphasis> |
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Authors: | Weifa Zheng Yanxia Zhao Xin Zheng Yubing Liu Shenyuan Pan Yucheng Dai Fuming Liu |
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Institution: | (1) Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou, 221116, China;(2) School of Life Sciences, Xuzhou Normal University, Xuzhou, 221116, China;(3) Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China;(4) Laboratory Center, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221009, China;(5) Present address: Illinois Institute of Technology, Chicago, IL, USA |
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Abstract: | While Inonotus obliquus produces a diverse range of bioactive metabolites in its natural habitats, it accumulates less in its submerged cultures.
We show here that coculture of I. obliquus with Phellinus punctatus resulted in less production of mycelial biomass but an increased accumulation of phenolic compounds, melanins, and lanostane-type
triterpenoids. Metabolites increased in production by coculture include phelligridin C, phelligridin H, methyl inoscavin A,
inoscavin C, inoscavin B, davallialactone, methyl davallialactone, foscoparianol D, 21,24-cyclopentalanosta-3β,21,25-triol-8-en,
lanosta-7,9(11),23-triene-3β,22,25-triol, and inotodisaccharide and melanins. Metabolites from coculture also showed an increased
potential for scavenging free radicals and inhibiting the proliferation of HeLa 229 cells. Davallialactone, methyl davallialactone,
and minor phenolic components are the major contributors for scavenging DPPH and hydroxyl radical in monoculture, and phelligridin
C, phelligridin H, methyl inoscavin A, inoscavin C, methyl davallialactone, foscoparianol D, and inotodisaccharide are those
for scavenging the tested radicals in coculture. Lanostane-type triterpenoids indicated limited roles in scavenging free radicals.
Nearly all the detected metabolites correlate positively with inhibiting proliferation of HeLa 229 cells. Thus, coculture
of I. obliquus with other fungi seems to be a cost-effective strategy for upregulating biosynthesis of bioactive metabolites. |
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