Comparative study on methyl orange removal by growing cells and washed cell suspensions of <Emphasis Type="Italic">Lactobacillus casei</Emphasis> TISTR 1500 |
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Authors: | Phisit Seesuriyachan Ampin Kuntiya Ken Sasaki Charin Techapun |
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Institution: | (1) Department of Biotechnology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100, Thailand;(2) Biomaterials Science and Engineering, Graduate School of Engineering, Hiroshima Kokusai Gakuin University, 6-20-1, Nakano, Akiku, Hiroshima 739-0321, Japan |
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Abstract: | Lactobacillus casei TISTR 1500 possesses cytoplasmic azoreductase, and converts methyl orange to N,N-dimethyl-p-phenylenediamine and 4-aminobenzenesulfonic acid. In culture growth, the strain completely degraded methyl orange at 200 mg/l,
even though the pH value was lower than 4. The decolorization was inhibited in the growing culture with 800 mg of the dye/l
after incubation for 12 h. The percentage of decolorization and specific decolorization rate with 400 and 800 mg/l were 66
and 15%, and 14.2, and 8.7 mg/gCell/h, respectively. Additionally, a growing culture is more tolerant to a high initial dye
concentration than when using washed cell suspensions supplied with only sucrose. Moreover, incubation of a low cell density
in 600 μM of Na+ and 20 mM of sucrose increased the specific decolorization rate from 2.34 mg/gCell/h (without Na+) to 4.32 mg/gCell/h. However, Na+ had no effect on the enhancement of azoreductase activity in the reaction mixture. |
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Keywords: | Lactobacillus casei Decolorization Metal ions Methyl orange Azoreductase |
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