Formation and identification of trimethylimidazole during tetramethylpyrazine production from glucose by <Emphasis Type="Italic">Bacillus</Emphasis> strains |
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| Authors: | Zijun Xiao Jian R Lu Cuiqing Ma Ping Xu |
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| Institution: | (1) Center for Bioengineering &; Biotechnology, China University of Petroleum, Qingdao, 266555, People’s Republic of China;(2) Biological Physics Group, School of Physics and Astronomy, The University of Manchester, Schuster Building, Manchester, M13-9PL, UK;(3) State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People’s Republic of China;(4) MOE Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China; |
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| Abstract: | During 2,3,5,6-tetramethylpyrazine production from glucose by Bacillus strains, a novel product was detected and identified as 2,4,5-trimethylimidazole (TMI) by GC/MS. TMI appeared in the culture
medium only after glucose had been depleted and then increased to 0.25–0.31 g l−1 in 90–120 h. When the ammonium source was changed from (NH4)2SO4 to (NH4)2HPO4, only about one tenth of TMI was detected. Although the mechanistic events largely remain unclear, both microbial strains
tested demonstrated similar dynamic processes of TMI production, suggesting that TMI formation is a genuine feature of Bacillus species.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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| Keywords: | Bacillus Identification Metabolite Trimethylimidazole |
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