Printing protein arrays from DNA arrays |
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Authors: | He Mingyue Stoevesandt Oda Palmer Elizabeth A Khan Farid Ericsson Olle Taussig Michael J |
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Institution: | Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK. |
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Abstract: | We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins. |
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