Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain |
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| Authors: | Nair Dhanalakshmi Memmi Guido Hernandez David Bard Jonathan Beaume Marie Gill Steven Francois Patrice Cheung Ambrose L |
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| Affiliation: | 1Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03755;2Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, Rue Gabrielle-Perret-Gentil, CH-1211, Geneva 14, Switzerland;3New York State Center of Excellence for Bioinformatics and Life Sciences, 701 Ellicott Street, Buffalo, New York 14203;4Department of Microbiology, University of Rochester, Rochester, New York 14620 |
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| Abstract: | Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies. |
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