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1,1-Dimethyl-4-(phenylsulfonyl)semicarbazide (DPSS) does not inhibit the in vitro activities of 1-aminocyclopropane-1-carboxylate (ACC) oxidase and ACC synthase obtained from senescing carnation Dianthus caryophyllus L.) petals
Authors:Shigeru Satoh  Naoko Oyamada  Toshihito Yoshioka  Naoki Midoh
Institution:(1) Laboratory of Bioadaptation, Graduate School of Agriculture, Tohoku University, Tsutsumidori-amamiyamachi 1-1, Aoba-ku, Sendai, 981, Japan;(2) Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd., Morooka-cho 760, Kohoku-ku, Yokohama, 222, Japan;(3) Present address: Miyagi Horticultural Experimental Station, Natori, Miyagi, 982-12, Japan
Abstract:The effects of 1,1-dimethyl-4-(phenylsulfonyl)semicarbazide (DPSS) on the in vitro activities of 1-aminocyclopropane-1-carboxylate (ACC) oxidase and ACC synthase isolated from senescing carnation petals were investigated. In contrast to a previous proposal, DPSS at 1 mM did not inhibit the in vitro activity of ACC oxidase. It was confirmed that DPSS does not inhibit ACC synthase activity. DPSS probably does not exert its inhibitory action on ethylene production by a direct action on ACC oxidase and ACC synthase, but by some unknown action.
Keywords:1-aminocyclopropane-1-carboxylate (ACC) oxidase  ACC synthase  carnation  Dianthus caryophyllus  1  1-dimethyl-4-(phenylsulfonyl)semicarbazide (DPSS)
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