| 尿蛋白膜保存法与直接冻存法的成本效益分析 |
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| 引用本文： | 王明铭,刘雪姣,贾露露,孙颖,高友鹤,李明喜. 尿蛋白膜保存法与直接冻存法的成本效益分析[J]. 生物工程学报, 2014, 30(7): 1128-1133 |
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| 作者姓名： | 王明铭 刘雪姣 贾露露 孙颖 高友鹤 李明喜 |
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| 作者单位： | 1 中国医学科学院 北京协和医学院 北京协和医院 肾内科，北京 100730；2 河北省邢台市人民医院 肾内科，河北 邢台 054000;1 中国医学科学院 北京协和医学院 北京协和医院 肾内科，北京 100730；3 首都医科大学附属北京安贞医院 肾内科，北京 100029;4 首都医科大学附属北京儿童医院，北京 100045；5 中国医学科学院 北京协和医学院 基础医学研究所 病理生理学系，北京 100005;1 中国医学科学院 北京协和医学院 北京协和医院 肾内科，北京 100730;5 中国医学科学院 北京协和医学院 基础医学研究所 病理生理学系，北京 100005;1 中国医学科学院 北京协和医学院 北京协和医院 肾内科，北京 100730 |
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| 基金项目： | 卫生公益性行业科研专项基金 (No. 200702008)，十二五国家科技支撑计划 (No. 2012-2015) 资助。 |
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| 摘 要： | 为了比较尿液蛋白PVDF膜富集保存法(尿膜)和尿液直接冻存法两种方法的优缺点。通过比较两种方法在时间、所占空间、费用、蛋白降解程度及大样本临床实践性方面的区别。发现在所占空间、电费方面及临床实践性方面尿膜保存法优于直接冻存法,而在时间及耗材花费方面直接冻存法优于尿膜保存法。因此尿蛋白的尿膜保存法比直接冻存法有更强的实际应用价值。
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| 关 键 词： | 尿液蛋白 富集保存方法 成本效益分析 |
| 收稿时间： | 2014-02-26 |
Comparison of cost-effectiveness between urimem and direct freezing for urinary protein preservation |
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| Mingming Wang,Xuejiao Liu,LuluJi,Ying Sun,Youhe Gao and Mingxi Li. Comparison of cost-effectiveness between urimem and direct freezing for urinary protein preservation[J]. Chinese journal of biotechnology, 2014, 30(7): 1128-1133 |
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| Authors: | Mingming Wang Xuejiao Liu LuluJi Ying Sun Youhe Gao Mingxi Li |
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| Affiliation: | 1 Department of Nephrology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100730, China; 2 Department of Nephrology, Xingtai People's Hospital, Xingtai 054000, Hebei, China;1 Department of Nephrology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100730, China; 3 Department of Nephrology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China;4 Beijing Children's Hospital, Capital Medical University, Beijing 100045, China; 5 Department of Physiology and Pathophysiology, National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100005, China;1 Department of Nephrology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100730, China;5 Department of Physiology and Pathophysiology, National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100005, China;1 Department of Nephrology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100730, China |
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| Abstract: | To compare two enrichment and preservation methods of urinary proteins, stored in polyvinylidene difluoride (PVDF) membrane (Urimem) or direct freezing, we examined the differences between the two methods in time, space, costs of supplies and electricity, degree of protein degradation and convenience of the sample handling. The urimem method is superior in the storage space, the cost of electricity and the clinical convenience compared to the direct freezing method. However, the direct freezing method is superior in the time and the cost of supplies to the urimem method. The enrichment and preservation of urinary proteins using urimem have more cost-effective benefits compared to those of the direct freezing method. |
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| Keywords: | urine proteins enrichment and preservation methods cost-effective analysis |
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