Protective effect of aquacultured flounder fish-derived peptide against oxidative stress in zebrafish |
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| Institution: | 1. Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea;2. Industry-Academy Cooperation Foundation, Jeju National University, Jeju 690-756, Republic of Korea;3. Department of Seafood Science and Technology, Institute of Marine Industry, Gyeongsang National University, Tongyeong 650-160, Republic of Korea;4. Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus) Pukyong National University, Busan 608-737, Republic of Korea;1. Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea;2. Marine Natural Products Laboratory, Korea Ocean Research & Development Institute, P.O. Box 29, Ansan 426-744, Republic of Korea;3. Laboratory of Natural Products Chemistry, Institute for Tropical Biology and Conservation, University Malaysia Sabah, Kota Kinabalu 88440, Sabah, Malaysia;1. College of Pharmaceutical Sciences, Southwest University, Chongqing, 400716, China;2. School of Life Sciences, Southwest University, Chongqing, 400715, China;3. Chongqing Productivity Promotion Centre for the Modernization of Chinese Medicine, Chongqing, 400716, China;4. Chongqing Engineering Research Center for Pharmaceutical Process and Quality Control, Chongqing, 400716, China;1. Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea;2. Marine Bio Research Team, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea;3. Department of Polymer Science and Engineering, Sunchon National University, Jeonnam 540-742, Republic of Korea;4. Industry-Academy Cooperation Foundation, Jeju National University, Jeju 690-756, Republic of Korea;5. Department of Biomedical Engineering, Pukyong National University, Busan 608-737, Republic of Korea;6. Aqua Green Technology Co. Ltd., 209 Jeju Bio-Industry Center, 102 Jejudaehakno, Jeju 690-121, Republic of Korea |
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| Abstract: | This study investigates the protective effect of aquacultured flounder fish-derived peptide (AFFP) against 2,2-azobis-(2-amidinopropane) hydrochloride (AAPH)-induced oxidative damage in a zebrafish model. Zebrafish embryos were evaluated for the protective effect by heartbeat rate, survival rate, ROS generation, lipid peroxidation, and cell death. In the results, the AAPH group showed a low survival rate, whereas the AFFP and AAPH co-treated group increased a survival rate. Also, AFFP dose-dependently reduced AAPH-induced intracellular ROS and lipid peroxidation, and decreased cell death in AAPH-induced zebrafish. These results revealed that AFFP could be used as a natural antioxidant, and that the zebrafish provides an alternative in vivo model to efficiently evaluate the antioxidative effects of peptides on fishes. |
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| Keywords: | Zebrafish Oxidative stress Antioxidative peptide AAPH |
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