Molecular cloning and expression analysis of two lipopolysaccharide-induced TNF-α factors (LITAFs) from rock bream,Oplegnathus fasciatus |
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| Institution: | 1. Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea;2. School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea;3. Pathology Division, National Fisheries Research and Development Institute, Busan 619-900, Republic of Korea;4. Oil & POPs Research Group, Korea Institute of Ocean Science & Technology, 391 Jangbuk-Ri, Jangmok-Myon, Geoje 656-834, Republic of Korea;1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. Graduate University of the Chinese Academy of Sciences, Beijing 100049, China;1. Key Laboratory of Fishery Drug Development of Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510380, Guangzhou, Guangdong, China;2. German Reference Laboratory for KHVD, Institute of Infectology, Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, 17493, Greifswald, Insel Riems, Germany;3. Key Laboratory of Recreational Fisheries, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510380, Guangzhou, Guangdong, China;1. Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea;2. School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea;3. Aquatic Life Disease Control Division, National Fisheries Research and Development Institute (NFRDI), 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea;4. Biotechnology Research Division, NFRDI, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea;1. Department of Aqualife Medicine, Chonnam National University, Chonnam 550-749, Republic of Korea;2. Aquatic Animal Hospital, Chonnam National University, Chonnam 550-749, Republic of Korea;3. Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea;4. National Fisheries Research and Development Institute, Busan, Republic of Korea;5. Genetic & Breeding Research Center, National Fisheries Research & Development Institute, Geoje 656-842, Republic of Korea;1. State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, College of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China;2. Department of Obstetrics and Gynecology, First Hospital Affiliated to the Fourth Military Medical University, Xi''an, 710032, China |
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| Abstract: | Lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-α factor (LITAF) plays an important role controlling the expression of TNF-α and the other cytokine genes in the presence of LPS. However, two LITAF homologues have not been characterized in fish. In this study, we cloned two distinct LITAF (RbLITAF1 and RbLITAF2) cDNAs from rock bream (Oplegnathus fasciatus) and characterized their expression profiles after infection with Edwardsiella tarda, Streptococcus iniae or red seabream iridovirus (RSIV). The coding regions of RbLITAF1 and RbLITAF2 cDNAs were 492 bp and 417 bp, encoding 153 and 138 amino acid residues, respectively. The genes consisted of a LITAF domain. RbLITAF1 was highly expressed in the spleen and heart of healthy rock bream, whereas RbLITAF2 was highly expressed in the gill, intestine and stomach. In spleen, the gene expression of RbLITAF1 and RbLITAF2 were increased until 5 days post-infection (dpi), and then decreased at 7 dpi. In kidney, E. tarda and RSIV infection led to induction of the RbLITAF1 gene at 1 dpi, RbLITAF2 gene was down-regulated after pathogen infection. These results suggest that RbLITAFs may be involved in the LITAF-mediated immune response and regulate systemic immune responses against pathogen infection. |
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| Keywords: | LITAF Red seabream iridovirus |
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