Characterization of Argonaute2 gene from black tiger shrimp (Penaeus monodon) and its responses to immune challenges |
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| Affiliation: | 1. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China;2. College of Animal Science, South China Agriculture University, Guangzhou 510642, PR China;1. Aquaculture Genomics Research Unit, Faculty of Biosciences and Aquaculture, University of Nordland, Bodø 8049, Norway;2. Institute of Marine Research, Bergen 5817, Norway;1. National Centre for Marine Conservation and Resource Sustainability, Australian Maritime College, University of Tasmania, Launceston, Tasmania, Australia;2. Fisheries Laboratory, Kinki University, Nishimuro, Wakayama, Japan;1. Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur, Chennai 603 203, Tamil Nadu, India;2. Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A4800, Austin, TX 78712, USA;3. Lab PCN 206, Microbiology Division, CSIR – Central Drug Research Institute, B.S. 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031, Uttar Pradesh, India;4. Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Semeling Bedong, 08100 Bedong, Kedah, Malaysia;1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, Guangdong, China;2. University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China;3. School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China;1. Developmental Cellomics Laboratory, Genome Institute of Singapore, Singapore 138672;2. Department of Biological Sciences, National University of Singapore, Singapore 117543 |
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| Abstract: | Argonaute2 binds to a short guide RNA (microRNA or short interfering RNA) and guides RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. Here we identified and characterized Argonaute2 from black tiger shrimp Penaeus monodon (designated as PmAgo2). The full-length cDNA of PmAgo2 contained a 5′ untranslated region (UTR) of 106 bp, an open reading frame (ORF) of 2616 bp and a 3′ UTR of 123 bp. The predicted PmAgo2 protein is 99.4 KDa with the theoretical isoelectric point of 9.54. PmAgo2 shared the highest similarity of amino acid with Marsupenaeus japonicus Argonaute2 and Litopenaeus vannamei Argonaute2, at 69.0% and 68.5%, respectively. Phylogenic analysis showed PmAgo2 clustered with shrimp Argonaute2, and closed to the group of insects. Real-time quantitative PCR showed that PmAgo2 was widely expressed in almost all examined tissues except eyestalk, with high expression in lymph and haemocyte. mRNA expression also revealed that PmAgo2 was significantly up-regulated by Staphylococcus aureus and White Spot Syndrome Virus (WSSV) in hepatopancreas. Furthermore, our study also confirmed that dsRNA and ssRNA homologous poly (I:C) and R848 activated the expression of PmAgo2. The result indicated that PmAgo2 responded to both bacterial infection and viral infection, especially, it may induce an ssRNA-mediated RNAi with other core members of siRNA pathway in black tiger shrimp. |
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| Keywords: | Argonaute2 RNAi pathway Bacteria Virus |
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