Vaccination of channel catfish with extracellular products of Aeromonas hydrophila provides protection against infection by the pathogen |
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| Affiliation: | 1. Norwegian College of Fishery Science, University of Tromsø, Breivika, N-9037 Tromsø, Norway;2. Centre for Organelle Research, Faculty of Science and Technology, University of Stavanger, 4021 Stavanger, Norway;3. Nofima, Box 6122, N-9291 Tromsø, Norway;1. Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, United Kingdom;2. Faculty of Veterinary Sciences, Mahidol University, Salaya Campus, Nakornpathom 73170, Thailand;3. Marine Scotland Science, Aberdeen AB11 9DB, Scotland, United Kingdom;4. Novus International, Novus Aqua Research Center, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Viet Nam;1. Aquaculture Biosecurity, LLC, Post Office Box 24, Auburn, AL 36831, USA;2. USDA:APHIS:VS, 2150 Centre Ave, Fort Collins, CO 80526, USA;3. University of West Alabama, Hwy 100, Livingston, AL 35470, USA;4. Auburn University, School of Fisheries, Aquaculture and Aquatic Sciences, 203 Swingle Hall, Auburn, AL 36849, USA;1. Fish Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751002, India;2. Gene Regulation Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110 067, India;3. Gene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India |
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| Abstract: | Aeromonas hydrophila, a Gram-negative bacterium, is one of the economically-important pathogens in modern aquaculture. Among various traits, extracellular products (ECP) secreted by the bacterium are considered to be essential factors for virulence. Whether vaccination with the ECP could produce immune protection in catfish against the pathogen was determined in this study. The results showed that fish vaccinated with ECP had 100% of relative percent survival (RPS) when challenged with the pathogen two weeks post vaccination. The anti-ECP serum from vaccinated fish could aggregate cells of homogeneous bacteria as well as other virulent strains (isolates) of A. hydrophila but not an A. veronii isolate and a low virulent field isolate. The agglutination titers increased from two weeks to four weeks post immunization and sustained a high level at week seven when the RPS remained at 100%. The anti-ECP serum could also provide naïve fish with immediate protection against A. hydrophila as evidenced by passive immunization. Immunoblotting analysis showed that the anti-ECP serum contained antibodies that bound to specific targets, including protein and lipopolysaccharide-like molecules, in the ECP. Mass spectrometric analysis identified following putative proteins that may serve as important immunogens: chitinase, chitodextrinase, outer membrane protein85, putative metalloprotease, extracellular lipase, hemolysin and elastase. Findings revealed in this study suggest that, while ECP prepared in a conventional and convenient way could be a vaccine candidate, further characterization of antibody-mediated targets in the ECP would uncover quintessential antigens for the future development of highly efficacious vaccines. |
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| Keywords: | Extracellular products Vaccination Agglutination Passive immunization |
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