Gene structure,immune response and evolution: Comparative analysis of three 2-Cys peroxiredoxin members of miiuy croaker,Miichthys miiuy |
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| Affiliation: | 1. Laboratoire de Génie Electrique et Ferroélectricité (LGEF), INSA LYON, Bat. Gustave Ferrie, Villeurbanne Cedex 69621, France;2. DAC HR Laboratory, Université Ferhat Abbas, Sétif 19000, Algeria;3. Ecole Nationale des Sciences Appliquées, Université Chouaib Doukkali, El Jadida, Morocco;4. Ecole Normale Supérieure Casablanca, Université Hassan II Casablanca, Morocco;5. Academy Hassan II of Science and Technology, Rabat, Morocco;1. Key Lab of Power Electronics for Energy Conservation, Motor Drive of Hebei Province, Yanshan University, Qinhuangdao, Hebei 066004, China;2. Qinhuangdao Power Corporation Limited, Qinhuangdao 066004, Hebei Province, China;3. Shijiazhuang Power Supply Corporation, Shijiazhuang 050000, Hebei Province, China;1. Department of Breast and Oncoplastic Surgery, Royal Surrey County Hospital, Guildford, UK;2. Department of Histopathology, Royal Surrey County Hospital, Guildford, UK;3. FHMS, University of Surrey, Guildford, UK;1. Sanifit Laboratoris S.L. Research and Development Department, 07121 Palma de Mallorca, Spain;2. Laboratory of Renal Lithiasis Research, Institute of Health Sciences Research (IUNICS), University of Balearic Islands, 07122 Palma of Mallorca, Spain |
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| Abstract: | Peroxiredoxin family was a superfamily of selenium independent peroxidases. It was divided into six subtypes: Prx1-4 (typical 2-Cys), Prx5 (atypical 2-Cys) and Prx6 (1-Cys). This study reports the isolation and characterization three 2-Cys peroxiredoxin members of full cDNA and genomic clones from miiuy croaker (Miichthys miiuy). The genetic structure analysis showed that the C-terminal catalytic Cys positioned within GEVCPAXW. This sequence was different between Prx3 and Prx4, but was conservative in different species of the same gene, the X base was S in Prx3 but G in Prx4. Tissues expression analysis showed that the expressions of Prx3 in liver and brain were much higher than other tissues; the values of Prx4 in spleen, intestine and kidney were significantly higher than others; and the expression of Prx5 in muscle was higher than that of other tissues. Real-time PCR results showed that there were highest values of these three Prxs emerging with the time post challenge of Vibrio anguillarum in liver, spleen and kidney although the highest value time differed from each other and the expression of these three genes also changed with the change of infection time. These results indicated that expression analysis of these three genes play some positive function against pathogenic bacteria infection in miiuy croaker. |
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| Keywords: | Peroxiredoxin Miiuy croaker Gene structure Expression profiling |
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