Somatic embryogenesis in cell suspension cultures of Acacia sinuata (Lour.) Merr. |
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Authors: | G?Vengadesan Email author" target="_blank">A?GanapathiEmail author V?Ramesh?Anbazhagan R?Prem?Anand |
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Institution: | (1) Department of Biotechnology, School of Life Sciences, Bharathidasan University, 620 024 Tiruchirapalli, Tamilnadu, India |
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Abstract: | Summary Suspension cultures initiated from calluses derived from seedling leaf explants of Acacia sinuata (Lour.) Merr. produced somatic
embryos. Embryogenic callus was induced on semisolid MS (Murashige and Skoog, 1962) medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic
acid (2,4-D) and 2.22 μM 6-benzylaminopurine. A high frequency of somatic embryos was induced in MS liquid medium supplemented
with 4.52 μM 2,4-D and 10% coconut water. Further studies on ontogeny of somatic embryos showed that the cells destined to
become somatic embryos divided into spherical proembryos. Subsequent development led to the formation of globular, heart,
torpedo-shaped and cotyledonary-stage embryos. The conversion of somatic embryos occurred on auxin-free MS medium. Effects
of various auxins, cytokinins, carbohydrates and amino acids in enhancing productin, of somatic embryos were studied. MS medium
supplemented with 87.64 mM sucrose and 342.46 μM glutamine promoted higher somatic embryo production whereas cytokinin had
no effect and led to recallusing of embryos. About 8–10% of embryos converted into plants. |
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Keywords: | callus shikakai somatic embryo growth regulator carbohydrates amino acids |
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