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Genetic engineering of wheat for increased resistance to powdery mildew disease
Authors:M. Bliffeld  J. Mundy  I. Potrykus  J. Fütterer
Affiliation:Institute of Plant Sciences, ETH Zürich, Universit?tstr. 2, CH 8092 Zürich, Switzerland Fax: +41-1-632 1044 E-mail: johannes.fuetterer@ipw.biol.ethz.ch, CH
Department of Plant Physiology, Institute of Molecular Biology, Oester Farimagsgade 2A, 1353 Copenhagen K, Denmark, DK
Abstract: Fungal wheat (Triticum aestivum) diseases greatly affect crop productivity and require the economically and ecologically undesirable application of fungicides in wheat agriculture. We have generated transgenic wheat plants constitutively expressing an antifungal barley-seed class II chitinase. The transgene was stably expressed and the chitinase properly localized in the apoplast of the transgenic lines. The engineered wheat plants showed increased resistance to infection with the powdery mildew-causing fungus Erysiphe graminis. Received: 20 October 1998 / Accepted: 26 October 1998
Keywords:  Antifungal proteins  Chitinase  Biolistic transformation  Wheat  Chlorophenolred  Phosphinotricin  Erysiphe graminis
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